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This application is a continuation-in-part of U. The present invention is in the field of molecular biology. More specifically, the invention is in the field of assays that utilize oligonucleotides as primers or hybridization probes. Minor groove binding agents which non-covalently bind into the minor groove of double stranded DNA are known in the art.
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One Direction Harry's big sister Gemma makes her modelling debut

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One Direction Harry's big sister Gemma makes her modelling debut | Daily Mail Online

Louis Tomlinson's mother has seen her son's love child in the womb and is 'getting on great' with the girl pregnant by the One Direction star. Johannah Deakin — who has seven children including Louis as well as one-year-old twins — met Californian stylist Briana Jungwirth earlier this month. Deakin traveled with her husband, Dan, to California and both sets of parents attended Briana's most recent ob-gyn appointment in Los Angeles. They all saw the ultrasound together and heard the baby's heart beat for the first time. New arrival: According to a mutual friend, the couple's baby is due early next year.
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